When repeats drive the vocabulary: a Byte-Pair Encoding analysis of T2T primate genomes

The emergence of telomere-to-telomere (T2T) genome assemblies has opened new avenues for comparative genomics, yet effective tokenization strategies for genomic sequences remain underexplored. In this pilot study, we apply Byte Pair Encoding (BPE) to nine T2T primate genomes including three human assemblies by training independent BPE tokenizers with a fixed vocabulary of 512,000 tokens using our custom tool, dnaBPE. Our analysis reveals that only 11,569 tokens are shared across all assemblies, while nearly 991,854 tokens are unique to a single genome, indicating a rapid decline in shared vocabulary with increasing assembly comparisons. Moreover, phylogenetic trees derived from token overlap failed to recapitulate established primate relationships, a discrepancy attributed to the disproportionate influence of species-specific high-copy repetitive elements. These findings underscore the dual nature of BPE tokenization: while it effectively compresses repetitive sequences, its sensitivity to high-copy elements limits its utility as a universal tool for comparative genomics. We discuss potential hybrid strategies and repeat-masking approaches to refine genomic tokenization, emphasizing the need for domain-specific adaptations in the development of large-scale genomic language models. The dnaBPE tool used in this study is open-source and available atthis https URL.

@article{popova2025_2505.08918,
  title={ When repeats drive the vocabulary: a Byte-Pair Encoding analysis of T2T primate genomes },
  author={ Marina Popova and Iaroslav Chelombitko and Aleksey Komissarov },
  journal={arXiv preprint arXiv:2505.08918},
  year={ 2025 }
}